Product Description
The CleanPlex® Hereditary Renal / Urinary Cancer Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing (NGS) assay designed to examine the germline variants or mutations across 28 genes associated with Hereditary Renal / Urinary Cancer. The panel targets all the exonic regions of those genes and the flanking intronic sequences. Compatible with just 10 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The pre-designed panel is optimized in silico to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.
This product is made to order. Once we receive your order, we will synthesize the panel and the kit will contain CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads can be ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.
Storage Temperature
Store at -20 °C.
For Research Use Only. Not for use in diagnostic procedures.
Gene List:
BAP1, CDC73, CDKN1C, DICER1, DIS3L2, EPCAM, FH, FLCN, GPC3, MET, MITF, MLH1, MSH2, MSH6, PMS2, PTEN, REST, SDHA, SDHB, SDHC, SDHD, SMARCA4, SMARCB1, TP53, TSC1, TSC2, VHL, WT1
References:
Hartnan et al. 2009. The role of the Birt-Hogg-Dubé protein in mTOR activation and renal tumorigenesis.
Coleman et al. Hereditary and familial kidney cancer. 2009. PubMed ID: 19584731
Orlova et al. The tuberous sclerosis complex. 2010. PubMed ID: 20146692
Maher et al. von Hippel-Lindau disease: a clinical and scientific review. 2011. PubMed ID: 21386872
Lubas et al. Exonuclease hDIS3L2 specifies an exosome-independent 3′-5′ degradation pathway of human cytoplasmic mRNA. 2013. PubMed ID: 23756462